Premium
Calcium channels in chicken sperm regulate motility and the acrosome reaction
Author(s) -
Nguyen Thi Mong Diep,
Duittoz Anne,
Praud Christophe,
Combarnous Yves,
Blesbois Elisabeth
Publication year - 2016
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.13710
Subject(s) - acrosome reaction , microbiology and biotechnology , voltage dependent calcium channel , sperm , calcium channel , gamete , biology , acrosome , ionomycin , calcium signaling , calcium , sperm motility , chemistry , signal transduction , motility , intracellular , genetics , organic chemistry
Intracellular cytoplasmic calcium ([Ca 2+ ] i ) has an important regulatory role in gamete functions. However, the biochemical components involved in Ca 2+ transport are still unknown in birds, an animal class that has lost functional sperm‐specific CatSper channels. Here, we provide evidence for the presence and expression of various Ca 2+ channels in chicken sperm, including high voltage‐activated channels (L and R types), the store‐operated Ca 2+ channel ( SOC ) component Orai1, the transient receptor potential channel ( TRPC 1) and inositol‐1,4,5–trisphosphate receptors ( IP 3 R1). L‐ and R–type channels were mainly localized in the acrosome and the midpiece, and T–type channels were not detected in chicken sperm. Orai1 was found in all compartments, but with a weak, diffuse signal in the flagellum. TRCP 1 was mainly localized in the acrosome and the midpiece, but a weak diffuse signal was also observed in the nucleus and the flagellum. IP 3 R1 was mainly detected in the nucleus. The L–type channel inhibitor nifedipine, the R–type channel inhibitor SNX –482 and the SOC inhibitors MRS –1845, 2– APB and YM –58483 decreased [Ca 2+ ] i sperm motility and acrosome reaction capability, with the SOC inhibitors inhibiting these functions most efficiently. Furthermore, we showed that Ca 2+ ‐mediated induction of AMP‐activated protein kinase ( AMPK ) phosphorylation was blocked by SOC inhibition. Our identification of important regulators of Ca 2+ signaling in avian sperm suggests that SOC s play a predominant role in gamete function, whereas T–type channels may not be involved. In addition, Ca 2+ entry via SOC s appears to be the most likely pathway for AMPK activation and energy‐requiring sperm functions such as motility and the acrosome reaction.