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Tumor suppressor gene OSCP 1 / NOR 1 regulates apoptosis, proliferation, differentiation, and ROS generation during eye development of Drosophila melanogaster
Author(s) -
Huu Nguyen Tho,
Yoshida Hideki,
Yamaguchi Masamitsu
Publication year - 2015
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.13528
Subject(s) - gene knockdown , biology , microbiology and biotechnology , drosophila melanogaster , eye development , apoptosis , cell growth , phenotype , gene , genetics
OSCP 1/ NOR 1 (organic solute carrier partner 1/oxidored nitrodomain‐containing protein 1) is a known tumor suppressor protein. OSCP 1 has been reported to mediate transport of various organic solutes into cells; however, its role during development has not yet been addressed. Here we report the results of studies on dOSCP 1 (the Drosophila ortholog of hOSCP 1 ) to elucidate the role of OSCP 1/ NOR 1 during development. Knockdown of dOSCP 1 in the eye imaginal discs induced a rough‐eye phenotype in adult flies. This phenotype resulted from induction of caspase‐dependent apoptosis followed by a compensatory cell proliferation and generation of reactive oxygen species in eye imaginal discs. The induction of apoptosis appears to be associated with down‐regulation of the anti‐apoptotic Buffy gene and up‐regulation of the pro‐apoptotic Debcl gene. These effects of knockdown of dOSCP 1 lead to mitochondrial fragmentation, degradation, and a shortfall in ATP production. We also found that knockdown of dOSCP 1 causes a defect in cone cell and pigment cell differentiation in pupal retinae. Moreover, mutations in epidermal growth factor receptor pathway‐related genes, such as Spitz and Drk , enhanced the rough‐eye phenotype induced by dOSCP 1 knockdown. These results suggest that dOSCP 1 positively regulates the epidermal growth factor receptor signaling pathway. Overall, our findings indicate that dOSCP 1 plays multiple roles during eye development in Drosophila .