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miR‐330‐5p inhibits proliferation and migration of keratinocytes by targeting Pdia3 expression
Author(s) -
Kim BongKyu,
Yoo HyeIn,
Choi Keonwoo,
Yoon Sungjoo Kim
Publication year - 2015
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.13523
Subject(s) - microrna , keratinocyte , microbiology and biotechnology , biology , gene expression , cell cycle , apoptosis , regulation of gene expression , gene , cell culture , genetics
The micro RNA s (mi RNA s) are a class of small non‐coding RNA molecules that play important roles in cellular processes by regulating gene expression at the post‐transcriptional level. In skin biology, several mi RNA s have been shown to be associated with differentiation, migration and apoptosis of keratinocytes, and regulation of the hair cycle. Although the biological role of miR‐330‐5p has been reported in several cancers and cells, the function and molecular mechanism of miR‐330‐5p in skin keratinocytes have not been identified. In this study, we found that miR‐330‐5p inhibited the proliferation and migration of mouse keratinocytes. Among the candidate target genes of miR‐330‐5p searched using microarray analysis, we found that the expression of Pdia3 was directly regulated by miR‐330‐5p in the mouse keratinocyte. Moreover, inhibition of Pdia3 expression caused decreased proliferation and migration ability of mouse keratinocytes. Additionally, expressions of miR‐330‐5p and Pdia3 displayed an inverse correlation with respect to the hair cycle stage. These results indicated that regulation of Pdia3 expression by miR‐330‐5p is important in maintaining the hair cycle through regulation of the proliferation and migration capability of keratinocytes.