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Polysialylation takes place in granulosa cells during apoptotic processes of atretic tertiary follicles
Author(s) -
Kaese Miriam,
Galuska Christina E.,
Simon Peter,
Braun Beate C.,
CabreraFuentes Hector A.,
Middendorff Ralf,
Wehrend Axel,
Jewgenow Katarina,
Galuska Sebastian P.
Publication year - 2015
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.13519
Subject(s) - neural cell adhesion molecule , polysialic acid , microbiology and biotechnology , granulosa cell , gene isoform , apoptosis , cell adhesion , biology , chemistry , cell , ovarian follicle , ovary , medicine , endocrinology , biochemistry , gene
In the neuronal system, polysialic acid (polySia) is known to be involved in several cellular processes such as the modulation of cell–cell interactions. This highly negatively‐charged sugar moiety is mainly present as a post‐translational modification of the neural cell adhesion molecule ( NCAM ). More than 20 years ago, differently glycosylated forms of NCAM were detected in the ovaries. However, the exact isoform of NCAM , as well as its biological function, remained unknown. Our analysis revealed that granulosa cells of feline tertiary follicles express the polysialylated form of NCAM ‐140. Unexpectedly, polySia was only expressed in the granulosa layers of atretic follicles and not of healthy follicles. By contrast, only the un‐polysialylated form of NCAM was present on the membrane of granulosa cells of healthy follicles. To study a possible cellular function of polySia in feline follicles, a primary granulosa cell culture model was used. Interestingly, loss of polySia leads to a significant inhibition of apoptosis, demonstrating that polySia is involved during atretic processes in granulosa cells. Thus, polySia might not only directly influence regeneration processes as shown, for example, in the neuronal system, but also apoptosis.