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miR‐124 exerts tumor suppressive functions on the cell proliferation, motility and angiogenesis of bladder cancer by fine‐tuning UHRF 1
Author(s) -
Wang Xinsheng,
Wu Qiaoli,
Xu Bin,
Wang Pengfei,
Fan Weijia,
Cai Ying,
Gu Xinquan,
Meng Fanping
Publication year - 2015
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.13502
Subject(s) - vasculogenic mimicry , bladder cancer , carcinogenesis , microrna , angiogenesis , cancer research , metastasis , biology , cell growth , motility , cancer , downregulation and upregulation , cancer cell , microbiology and biotechnology , gene , biochemistry , genetics
UHRF 1, an epigenetic factor, is implicated in various cellular processes of tumorigenesis. However, the modulation of UHRF 1 expression in human bladder cancer at post‐transcriptional levels remains unclear. Here, we report that miR‐124 suppresses expression of UHRF 1 to affect the progression of human bladder cancer through competitive binding of the same region of its 3′‐ UTR . We show that compared with corresponding normal tissues, UHRF 1 is upregulated and miR‐124 is downregulated in bladder cancer tissues, demonstrating an inverse correlation of miR‐124 and UHRF 1. Quantitative PCR and western blot assay demonstrated that over‐expression of miR‐124 resulted in the suppression of UHRF 1. Furthermore, luciferase assay revealed that miR‐124 could control the fate of target gene UHRF 1 mRNA by binding 3′‐ UTR . The rescue experiment confirmed that miR‐124 exerted its biological functions by targeting UHRF 1. miR‐124 over‐expression significantly attenuated cellular proliferation, migration, invasion and vasculogenic mimicry in vitro , and tumor growth in vivo . UHRF 1 si RNA showed significant inhibitory effects on bladder cancer cells. Collectively, our study demonstrates that miR‐124 can impair the proliferation or metastasis of human bladder cancer cells by down‐regulation of UHRF 1.