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Cytokine‐induced macropinocytosis in macrophages is regulated by 14‐3‐3ζ through its interaction with serine‐phosphorylated coronin 1
Author(s) -
BoseDasgupta Somdeb,
Moes Suzette,
Jenoe Paul,
Pieters Jean
Publication year - 2015
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.13214
Subject(s) - pinocytosis , microbiology and biotechnology , phosphorylation , cytokine , biology , stimulation , kinase , receptor , immunology , biochemistry , neuroscience , endocytosis
The induction of macropinocytosis in macrophages during an inflammatory response is important for clearance of pathogenic microbes as well as the generation of appropriate immune responses. Recent data suggest that cytokine stimulation of macrophages induces macropinocytosis through phosphorylation of the protein coronin 1, thereby redistributing coronin 1 from the cell cortex to the cytoplasm followed by the activation of phosphoinositol‐3 ( PI ‐3) kinase. However, how coronin 1 phosphorylation regulates these processes remains unclear. We here define an essential role for 14‐3‐3ζ in cytokine‐induced and coronin‐1‐dependent macropinocytosis in macrophages. We found that, upon stimulation, phosphorylated coronin 1 transiently associated with 14‐3‐3ζ and receptor of activated C kinase 1 ( RACK 1). Importantly, downregulation of 14‐3‐3ζ, but not RACK 1, prevented relocation of coronin 1, as well as the induction of PI ‐3 kinase activity and thereby macropinocytosis upon cytokine stimulation. Together these data define an essential role for 14‐3‐3ζ in the regulation of macropinocytosis in macrophages upon cytokine stimulation through modulation of the localization of coronin 1.