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Structural basis of multivalent galactose‐based dendrimer recognition by human galectin‐7
Author(s) -
Ramaswamy Sneha,
Sleiman Mazen H.,
Masuyer Geoffrey,
ArbezGindre Cécile,
MichaScrettas Maria,
Calogeropoulou Theodora,
Steele Barry R.,
Acharya K. Ravi
Publication year - 2015
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.13140
Subject(s) - galectin , protein data bank (rcsb pdb) , protein data bank , galactose , glycobiology , chemistry , biochemistry , biology , computational biology , microbiology and biotechnology , protein structure , glycan , glycoprotein
Galectins are evolutionarily conserved and ubiquitously present animal lectins with a high affinity for β-galactose-containing oligosaccharides. To date, 15 mammalian galectins have been identified. Their involvement in cell-cell and cell-matrix interactions has highlighted their importance in signal transduction and other intracellular processes. Human galectin-7 (hGal-7) is a 15 kDa proto type galectin that forms a dimer in solution and its involvement in the stimulation and development of tumour growth has been reported. Previously, we reported the crystal structure of hGal-7 and its complex with galactose and lactose which provided insight into its molecular recognition and detailed interactions. Here, we present newly obtained high-resolution structural data on carbohydrate-based dendrons in complex with hGal-7. Our crystallographic data reveal how multivalent ligands interact with and form cross-links with these galectin molecules. Understanding how these dendrimeric compounds interact with hGal-7 would help in the design of new tools to investigate the recognition of carbohydrates by lectins.