Premium
Protein post‐translational modification in host defense: the antimicrobial mechanism of action of human eosinophil cationic protein native forms
Author(s) -
Salazar Vivian A.,
Rubin Jenny,
Moussaoui Mohammed,
Pulido David,
Nogués Maria Victòria,
Venge Per,
Boix Ester
Publication year - 2014
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.13082
Subject(s) - eosinophil cationic protein , posttranslational modification , host (biology) , antimicrobial , mechanism of action , mechanism (biology) , eosinophil , microbiology and biotechnology , chemistry , biology , immunology , biochemistry , in vitro , genetics , asthma , philosophy , epistemology , enzyme
Knowledge on the contribution of protein glycosylation in host defense antimicrobial peptides is still scarce. We have studied here how the post‐translational modification pattern modulates the antimicrobial activity of one of the best characterized leukocyte granule proteins. The human eosinophil cationic protein ( ECP ), an eosinophil specific granule protein secreted during inflammation and infection, can target a wide variety of pathogens. Previous work in human eosinophil extracts identified several ECP native forms and glycosylation heterogeneity was found to contribute to the protein biological properties. In this study we analyze for the first time the antimicrobial activity of the distinct native proteins purified from healthy donor blood. Low and heavy molecular weight forms were tested on Escherichia coli cell cultures and compared with the recombinant non‐glycosylated protein. Further analysis on model membranes provided an insight towards an understanding of the protein behavior at the cytoplasmic membrane level. The results highlight the significant reduction in protein toxicity and bacteria agglutination activity for heavy glycosylated fractions. Notwithstanding, the lower glycosylated fraction mostly retains the lipopolysaccharide binding affinity together with the cytoplasmic membrane depolarization and membrane leakage activities. From structural analysis we propose that heavy glycosylation interferes with the protein self‐aggregation, hindering the cell agglutination and membrane disruption processes. The results suggest the contribution of post‐translational modifications to the antimicrobial role of ECP in host defense.