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Epstein–Barr virus encoded micro RNA s target SUMO ‐regulated cellular functions
Author(s) -
Callegari Simone,
Gastaldello Stefano,
Faridani Omid R.,
Masucci Maria G.
Publication year - 2014
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.13040
Subject(s) - biology , microrna , microbiology and biotechnology , interactome , virus , viral replication , genetics , computational biology , gene
Post‐translational modification by the small ubiquitin‐like modifier ( SUMO ) regulates the cellular response to different types of stress and plays a pivotal role in the control of oncogenic viral infections. Here we investigated the capacity of micro RNA s (mi RNA s) encoded by Epstein–Barr virus to interfere with the SUMO signaling network. Using a computational strategy that scores different properties of mi RNA –m RNA target pairs, we identified a minimal set of 575 members of the SUMO interactome that may be targeted by one or more Epstein–Barr virus mi RNA s. A significant proportion of the candidates cluster in a functional network that controls chromatin organization, stress, DNA damage and immune responses, apoptosis and transforming growth factor beta signaling. Multiple components of the transforming growth factor beta signaling pathway were inhibited upon upregulation of the Bam HI ‐H rightward open reading frame 1 ( BHRF 1) encoded mi RNA s in cells transduced with recombinant lentiviruses or entering the productive virus cycle. These findings point to the capacity of viral mi RNA s to interfere with SUMO ‐regulated cellular functions that control key aspects of viral replication and pathogenesis.