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Overexpression of juxtaposed with another zinc finger gene 1 reduces proinflammatory cytokine release via inhibition of stress‐activated protein kinases and nuclear factor‐κB
Author(s) -
Yang Mengliu,
Dai Jihuan,
Jia Yanjun,
Suo Langquzhen,
Li Shengbing,
Guo Yunsong,
Liu Hua,
Li Ling,
Yang Gangyi
Publication year - 2014
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.12853
Subject(s) - proinflammatory cytokine , kinase , tumor necrosis factor alpha , endocrinology , medicine , biology , protein kinase a , monocyte , microbiology and biotechnology , chemistry , inflammation , biochemistry
As an inhibitor of the nuclear receptor subfamily 2, group C, member 2 signaling pathway, juxtaposed with another zinc finger gene 1 ( JAZF 1) has been shown to be involved in gluconeogenesis, lipid metabolism, and insulin sensitivity. However, its role in hepatic lipogenesis and chronic low‐grade inflammation leading to nonalcoholic fatty liver disease remains unknown. The aim of this study was to examine whether JAZF 1 overexpression in vivo or in vitro can protect against palmitic acid ( PA )‐induced and high‐fat diet ( HFD )‐induced systemic inflammatory responses, and the potential mechanism of this process. JAZF 1 overexpression vector was transfected into PA‐treated IAR‐20 hepatocytes. The mRNA expression levels of proinflammatory cytokines were measured by real‐time quantitative PCR , and stress‐activated protein kinase activities were measured by immunoblotting. For in vivo studies, JAZF 1 transgenic mice were fed an HFD for 12 weeks. Liver tissue was obtained for histological examination, real‐time RT ‐ PCR , and western blot analysis. PA significantly increased the expression levels of tumor necrosis factor‐α, monocyte chemotactic protein‐1 and interleukin‐8 mRNA in IAR‐20 hepatocytes in a dose‐dependent and time‐dependent manner. Treatment with JAZF 1 or stress‐activated protein kinase inhibitors inhibited PA‐induced tumor necrosis factor‐α, monocyte chemotactic protein‐1 and interleukin‐8 expression in these cells. In JAZF 1‐treated cells, the decreased expression of proinflammatory cytokines was accompanied by decreased p38 mitogen‐activated protein kinase and c‐Jun N‐terminal kinase phosphorylation and increased nuclear factor‐κB inhibitor‐α protein levels, similarly to the role of signaling inhibitors. In vivo , HFD ‐induced expression of proinflammatory cytokines was markedly attenuated in JAZF 1‐Tg mice as compared with controls. This attenuation was accompanied by decreased activation of c‐Jun N‐terminal kinase, p38 mitogen‐activated protein kinase, and nuclear factor‐κB. These data provide evidence for the important role of JAZF 1 in preventing lipogenesis and systemic inflammation‐related disease.