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Activating transcription factor 4 regulates adipocyte differentiation via altering the coordinate expression of CCATT /enhancer binding protein β and peroxisome proliferator‐activated receptor γ
Author(s) -
Yu Kaifan,
Mo Delin,
Wu Ming,
Chen Hu,
Chen Luxi,
Li Mingsen,
Chen Yaosheng
Publication year - 2014
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.12792
Subject(s) - transcription factor , adipocyte , adipogenesis , microbiology and biotechnology , peroxisome proliferator activated receptor , enhancer , regulator , biology , adipose tissue , chemistry , receptor , endocrinology , biochemistry , gene
Adipose tissue is crucial for energy homeostasis and is a topic interest with respect to investigating the regulation of adipose tissue formation for the ever‐increasing health concerns of obesity and type 2 diabetes. Adipocyte differentiation is tightly regulated by the characteristic sequential expression change of adipocyte genes, including members of the CCATT /enhancer binding protein ( C / EBP ) family of transcription factors, peroxisome proliferator‐activated receptor γ and tribbles homolog 3. In the present study, we demonstrate that C / EBP β and peroxisome proliferator‐activated receptor γ (but not tribbles homolog 3) are targeted for activation by activating transcription factor 4 ( ATF 4), a member of c AMP response element‐binding/activator transcription factor family. Importantly, overexpression of ATF4 in 3T3‐L1 cells enhanced adipogenesis, whereas small‐interfering ATF4 blocked conversion of preadipocytes to adipocytes. These findings were accomplished by altering the coordinate expression of adipogenic transcription factors. Taken together, our results suggest that ATF 4 is a positive regulator of adipocyte differentiation. This notion is also supported by the results of the present study showing that the expression of ATF 4 is induced during adipocyte differentiation. Thus, ATF 4 could be an important regulator of energy homeostasis.