Premium
The GAR domain of GAS 2L3 mediates binding to the chromosomal passenger complex and is required for localization of GAS 2L3 to the constriction zone during abscission
Author(s) -
Fackler Marc,
Wolter Patrick,
Gaubatz Stefan
Publication year - 2014
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.12766
Subject(s) - cytokinesis , microbiology and biotechnology , abscission , mitosis , chemistry , midbody , biology , biophysics , cell division , cell , biochemistry , genetics
GAS 2L3 is a recently identified tubulin‐ and actin‐binding protein that regulates cytokinesis and abscission. In this study we show that GAS 2L3 interacts with the chromosomal passenger complex ( CPC ), which plays key roles in mitosis and cytokinesis. Biochemical assays show that GAS 2L3 directly interacts with the C‐terminus of borealin and the N‐terminus of survivin. We find that the interaction between these two CPC subunits and GAS 2L3 is mediated by the conserved GAR domain of GAS 2L3. We further show that the GAR domain of GAS 2L3 is required for localization of GAS 2L3 to the constriction zone. Taken together these data suggest that GAS 2L3 is a downstream effector of the CPC during cytokinetic abscission.