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An experimental study of the regulation of glycolytic oscillations in yeast
Author(s) -
Schrøder Tine D.,
Özalp Veli C.,
Lunding Anita,
Jernshøj Kit D.,
Olsen Lars F.
Publication year - 2013
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.12522
Subject(s) - phosphofructokinase , hexokinase , glycolysis , biology , gene isoform , gene , cytochrome c oxidase , atpase , biochemistry , enzyme , mitochondrion , saccharomyces cerevisiae , microbiology and biotechnology
We have studied oscillating glycolysis in the strain BY 4743 and isogenic strains with deletions of genes encoding enzymes in glycolysis, mitochondrial electron transport and ATP synthesis. We found that deletion of the gene encoding the hexokinase 1 isoform does not affect the oscillations while deletion of the gene encoding the hexokinase 2 isoform results in oscillations with smaller amplitude. The latter is associated with an almost 50% decrease in hexokinase activity. Deletions in the genes encoding the α‐ and β‐subunits of phosphofructokinase abolish the oscillations entirely. This loss in oscillatory activity is associated with a fourfold decrease in phosphofructokinase activity. Deletions of genes encoding subunits of the F 1 F 0 ATP ase also inhibit the oscillations in accordance with earlier studies using for example inhibitors. Finally, we identified an apparently new control point involving the mitochondrial cytochrome c oxidase. The latter is difficult to explain as oscillatory activity entails 100% inhibition of this enzyme. The mitochondria of this strain seem to have normal F 1 F 0 ATP ase activity. Overall these results support earlier experimental and model studies suggesting that in addition to processes within glycolysis also processes outside this pathway contribute to the control of the oscillatory behaviour.