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Resistin affects lipid metabolism during adipocyte maturation of 3T3‐L1 cells
Author(s) -
Ikeda Yoshito,
Tsuchiya Hiroyuki,
Hama Susumu,
Kajimoto Kazuaki,
Kogure Kentaro
Publication year - 2013
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.12514
Subject(s) - resistin , adipocyte , medicine , gene knockdown , endocrinology , lipid metabolism , small interfering rna , biology , 3t3 l1 , fatty acid binding protein , adipose tissue , chemistry , acetyl coa carboxylase , transfection , biochemistry , pyruvate carboxylase , insulin resistance , adipokine , gene , insulin , enzyme
Resistin, an adipose‐tissue‐specific secretory factor, aggravates metabolic syndrome through impairment of glucose metabolism. Previously, we demonstrated that resistin expression was induced in both 3T3‐L1 cells and primary pre‐adipocytes derived from Zucker obese rats during the process of differentiation and maturation (Ikeda Y, Hama S, Kajimoto K, Okuno T, Tsuchiya H & Kogure K (2011) Biol Pharm Bull 34 , 865–870). However, the biological function of resistin in adipocytes is poorly understood. In the present study, we examined the effects of resistin knockdown on the biological features of 3T3‐L1 cells. We found that lipid content was significantly decreased in 3T3‐L1 cells transfected with anti‐resistin small interfering RNA (si RNA ) after adipocyte differentiation. While expression of peroxisome proliferator activated receptor γ and CCAAT /enhancer‐binding protein α was not affected, protein expression and transcriptional activity levels of carbohydrate response element binding protein (Ch REBP ), which upregulates transcription of lipogenic genes, decreased after anti‐resistin si RNA treatment. Moreover, gene expression of fatty acid synthase and acetyl‐CoA carboxylase 2, which are known to be regulated by Ch REBP , were also suppressed by resistin knockdown. In contrast, activity of the fatty acid β‐oxidation‐regulating protein carnitine palmitoyltransferase 1 increased. These results suggest that resistin knockdown induces suppression of lipid production and activation of fatty acid β‐oxidation. Consequently, resistin may affect lipid metabolism during adipocyte maturation.