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Adiponectin protects L eydig cells against proinflammatory cytokines by suppressing the nuclear factor‐κ B signaling pathway
Author(s) -
Wu Ling,
Xu Bufang,
Fan Weimin,
Zhu Xiaobin,
Wang Guangyan,
Zhang Aijun
Publication year - 2013
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.12391
Subject(s) - adiponectin , proinflammatory cytokine , medicine , endocrinology , receptor , adiponectin receptor 1 , cytokine , signal transduction , adipokine , chemistry , microbiology and biotechnology , biology , inflammation , insulin , insulin resistance
Adiponectin is an adipocyte hormone that is predominantly secreted by adipocytes, and has important roles in glucose and lipid homeostasis. Recent studies have shown that adiponectin is also involved in the regulation of many endocrine organs, such as the ovary, adrenal gland, and pituitary. However, its biological role in male testes is largely unexplored. The present findings demonstrate the presence of adeponectin receptors (adiponectin receptor 1 and adiponectin receptor 2) in TM 3 cells derived from mouse Leydig cells. Proinflammatory cytokine treatment significantly downregulated m RNA and protein levels of adiponectin receptor 1 and adiponectin receptor 2. However, adiponectin pretreatment successfully inhibited the signaling pathway mediated by proinflammatory cytokines. At the molecular level, we provide compelling evidence that adeponectin achieves this by suppressing nuclear factor‐κB activation through promotion of AMP ‐activated protein kinase phosphorylation. Thus, our data clearly indicate that adiponectin plays a protective role in L eydig cells through its anti‐inflammatory actions.