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Thioredoxin h regulates calcium dependent protein kinases in plasma membranes
Author(s) -
UeokaNakanishi Hanayo,
Sazuka Takashi,
Nakanishi Yoichi,
Maeshima Masayoshi,
Mori Hitoshi,
Hisabori Toru
Publication year - 2013
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.12301
Subject(s) - thioredoxin , microbiology and biotechnology , biochemistry , cytosol , chemistry , kinase , protein kinase a , signal transduction , biology , oxidative stress , enzyme
Thioredoxin ( T rx) is a key player in redox homeostasis in various cells, modulating the functions of target proteins by catalyzing a thiol–disulfide exchange reaction. Target proteins of cytosolic T rx‐ h of higher plants were studied, particularly in the plasma membrane, because plant plasma membranes include various functionally important protein molecules such as transporters and signal receptors. Plasma membrane proteins from Arabidopsis thaliana cell cultures were screened using a resin T rx‐ h 1 mutant‐immobilized, and a total of 48 candidate proteins obtained. These included two calcium‐sensing proteins: a phosphoinositide‐specific phospholipase 2 ( A t PLC 2) and a calcium‐dependent protein kinase 21 ( A t CPK 21). A redox‐dependent change in A t CPK 21 kinase activity was demonstrated in vitro . Oxidation of A t CPK 21 resulted in a decrease in kinase activity to 19% of that of untreated A t CPK 21, but Trx‐ h 1 effectively restored the activity to 90%. An intramolecular disulfide bond ( C ys97– C ys108) that is responsible for this redox modulation was then identified. In addition, endogenous A t CPK 21 was shown to be oxidized in vivo when the culture cells were treated with H 2 O 2 . These results suggest that redox regulation of A t CPK 21 by T rx‐ h in response to external stimuli is important for appropriate cellular responses. The relationship between the redox regulation system and Ca 2+ signaling pathways is discussed. Structured digital abstractCPK21 physically interacts with TRXH1 by two hybrid ( View interaction ) TRXH1 physically interacts with AT1G56075 , CPK21 , PRX2B , HIR3 , RAA4B , TC132 , RAD2C , AT3G03270 , AT3G53990 , METK4 , AT3G17020 , AT1G20760 , MPCP3 , SKS2 , AT5G23890 , DTC , AT1G56070 , TBB3 , ACT8 , TI110 , DHSB1 , TBA3 , AOX1A , PLCD2 , CLAH1 , CLAH2 , RSSA1 , APX1 , RAB1C , ACT7 , ARF1 , NRL1 , ADT1 , TBB7 , RAD2A , G3PC1 , TBB4 , MD37E , DHSA1 , TC159 , AT4G22710 , GPX6 , RABC1 , VATA , GPX2 and RAA2A by affinity chromatography technology ( View interaction )