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H 2 ‐driven cofactor regeneration with NAD ( P ) + ‐reducing hydrogenases
Author(s) -
Lauterbach Lars,
Lenz Oliver,
Vincent Kylie A.
Publication year - 2013
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.12245
Subject(s) - cofactor , nad+ kinase , nicotinamide , chemistry , hydrogenase , enzyme , ralstonia , biochemistry , combinatorial chemistry
A large number of industrially relevant enzymes depend upon nicotinamide cofactors, which are too expensive to be added in stoichiometric amounts. Existing NAD ( P ) H ‐recycling systems suffer from low activity, or the generation of side products. H 2 ‐driven cofactor regeneration has the advantage of 100% atom efficiency and the use of H 2 as a cheap reducing agent, in a world where sustainable energy carriers are increasingly attractive. The state of development of H 2 ‐driven cofactor‐recycling systems and examples of their integration with enzyme reactions are summarized in this article. The O 2 ‐tolerant NAD + ‐reducing hydrogenase from R alstonia eutropha is a particularly attractive candidate for this approach, and we therefore discuss its catalytic properties that are relevant for technical applications.