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Micro RNA ‐15a promotes neuroblastoma migration by targeting reversion‐inducing cysteine‐rich protein with K azal motifs ( RECK ) and regulating matrix metalloproteinase‐9 expression
Author(s) -
Xin Chen,
Buhe Bao,
Hongting Lu,
Chuanmin Yang,
Xiwei Hao,
Hong Zhang,
Lulu Han,
Qian Dong,
Renjie Wang
Publication year - 2013
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/febs.12074
Subject(s) - reversion , ectopic expression , matrix metalloproteinase , untranslated region , neuroblastoma , chemistry , messenger rna , microbiology and biotechnology , microrna , cell culture , cancer research , biology , phenotype , gene , biochemistry , genetics
In this study, we found that the expression of mi R ‐15a was positively correlated with neuroblastoma ( NB ) clinical pathological stage and was negatively correlated with reversion‐inducing cysteine‐rich protein with Kazal motifs ( RECK ) expression. Using the enhanced green fluorescent protein ( EGFP ) reporter construct carrying the 3′‐ UTR of RECK , we identified RECK as a direct target of miR‐15a. Suppression of miR‐15a significantly decreased the migration ability of GI ‐ LA ‐N and SK ‐N‐ SH cell lines, whereas overexpression of miR‐15a increased the migration ability; these effects could be partly reversed by RECK inhibition or ectopic expression. Moreover, inhibition of miR‐15a significantly increased secreted matrix metalloproteinase‐9 expression in culture medium through regulating the expression of RECK . These findings provide new insights into the characteristics of the miR‐15a– RECK –matrix metalloproteinase‐9 axis in NB progression, especially in NB migration and invasion.