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The curative effect of cannabinoid 2 receptor agonist on functional failure and disruptive inflammation caused by intestinal ischemia and reperfusion
Author(s) -
Bayram Sait,
Parlar Ali,
Arslan Seyfullah Oktay
Publication year - 2020
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/fcp.12502
Subject(s) - agonist , inflammation , contractility , pharmacology , endocrinology , ileum , medicine , cannabinoid receptor type 2 , cannabinoid receptor , reperfusion injury , ischemia , receptor , chemistry
Abstract Ischemia and reperfusion of intestinal tissue (intestinal I/R) induce disruption of ileal contractility and chain responses of inflammatory. The aim of this study was to reveal whether therapeutic value of cannabinoid 2 ( CB 2) receptor activity in the intestinal I/R, via to the exogenous administration of CB 2 agonist ( AM ‐1241). Intestinal I/R injury were performed through 30‐min ischemia and 150‐min reperfusion of mesenteric artery in Wistar rats. The pre‐administered doses of 0.1, 1, and 5 mg/kg of CB 2 agonist were studied to inhibit inflammation of intestinal I/R injury including ileum smooth muscle contractility, polymorphonuclear cell migration, oxidant/antioxidant defense system, and provocative cytokines. Pre‐administration with CB 2 receptor agonist ensured to consider improving the disrupted contractile responses in ileum smooth muscle along with decreased the formation of MDA that production of lipid peroxidation, reversed the depleted glutathione, inhibited the expression of TNF ‐α and of IL ‐1β in the intestinal I/R of rats. Taken together results of this research, the agonistic activity of CB 2 receptor for healing of intestinal I/R injury is ensuring associated with anti‐inflammatory mechanisms such as the inhibiting of migration of inflammatory polymorphonuclear cells that origin of acute and initial responses of inflammation, the inhibiting of production of provocative and pro‐inflammatory cytokines like TNF ‐α and IL ‐1β, the rebalancing of oxidant/antioxidant redox system disrupted in injury of reperfusion period and the supporting of physiologic defensive systems in endothelial and inducible inflammatory cells.