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Growth inhibitory and proapoptotic effects of l ‐asparaginase from Fusarium culmorum ASP ‐87 on human leukemia cells (Jurkat)
Author(s) -
Meghavarnam Anil K.,
Salah Maryam,
Sreepriya Meenakshisundaram,
Janakiraman Savitha
Publication year - 2017
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/fcp.12257
Subject(s) - jurkat cells , apoptosis , dna laddering , fusarium culmorum , cell cycle , cell culture , microbiology and biotechnology , leukemia , biology , dna fragmentation , t cell leukemia , cell growth , programmed cell death , t cell , biochemistry , immunology , fusarium , immune system , genetics , horticulture
The objective of this study was to evaluate the anticancer properties of l ‐asparaginase purified from fungal isolate Fusarium culmorum ASP ‐87 against human T‐cell leukemia cell line (Jurkat). The growth inhibitory and proapoptotic effects of purified l ‐asparaginase on Jurkat cell lines were investigated by determining its influence on cell viability, colony formation, DNA fragmentation, and cell cycle progression. The results revealed that purified l ‐asparaginase showed significant decrease in cell survival with IC 50 value of 90 μg/mL (9 IU/mL). The enzyme inhibited colony formation and showed characteristic laddering pattern on agarose gel thereby confirming the induction of apoptosis. Further, cell cycle analysis revealed that the enzyme induced apoptotic cell death by arresting the growth of cells at G 2 ‐M phase. However, the enzyme did not elicit any toxic effects on human erythrocytes. l ‐asparaginase purified from F. culmorum ASP ‐87 showed significant and selective cytotoxic and apoptotic effects on human T‐cell leukemic cells in dose‐dependent manner. Results of the study give leads for the anticancer effects of fungal l ‐asparaginase and its potential usefulness in the chemotherapy of leukemia.