Premium
Advanced oxidation protein products induce apoptosis of human chondrocyte through reactive oxygen species‐mediated mitochondrial dysfunction and endoplasmic reticulum stress pathways
Author(s) -
Ye Wenbin,
Zhu Siyuan,
Liao Congrui,
Xiao Jun,
Wu Qian,
Lin Zhen,
Chen Jianting
Publication year - 2017
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/fcp.12229
Subject(s) - reactive oxygen species , endoplasmic reticulum , apoptosis , unfolded protein response , oxidative stress , chondrocyte , mitochondrion , microbiology and biotechnology , mitochondrial ros , chemistry , stimulation , biology , endocrinology , biochemistry , in vitro
Advanced oxidation production products (AOPPs) have been confirmed to accumulate in patients with rheumatoid arthritis (RA). Previous study demonstrated that AOPPs could accelerate cartilage destruction in rabbit arthritis model. However, the effect of AOPP stimulation on apoptosis of human chondrocyte and the underlying mechanisms remains unclear. This study demonstrated that exposure of chondrocyte to AOPPs resulted in cell apoptosis. AOPP stimulation triggered reactive oxygen species (ROS) production, which induced mitochondrial dysfunction and endoplasmic reticulum stress (ER stress) resulted in caspase activation. Furthermore, an antioxidant, N ‐acetylcysteine, markedly blocked these signals. Our study demonstrated that AOPPs induce apoptosis via ROS‐related mitochondria‐ and ER‐dependent signals in human chondrocyte. Targeting AOPP‐triggered ROS generation might be as a promising option for patients with RA.