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Analysis of the antiepileptic, ethosuximide impacts on neurogenesis of rat forebrain stem cells
Author(s) -
Sondossi Kiana,
Majdzadeh Maryam,
Ghaeli Padideh,
Ghahremani Mohammad Hossein,
Shafaroodi Hamed,
Paknejad Babak,
Ostad Seyed Nasser
Publication year - 2014
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/fcp.12061
Subject(s) - neurogenesis , forebrain , ethosuximide , neuroscience , psychology , epilepsy , anticonvulsant , central nervous system
Specific GABA ergic interneurons in the hilus are lost in animal models with temporal‐lobe epilepsy ( TLE ). Some preclinical evidence has indicated that GABA ergic cells may provide relief from seizures in these models. This study was aimed to examine the ability of ethosuximide, an anticonvulsant drug, to promote neurogenesis in 3‐day‐old rat forebrain cortex stem cells. Most of the cells were found to be nestin‐positive undifferentiated neural stem cells prior to their exposure to ethosuximide. It was noted that the number and percentage of tubulin β‐III immunopositive neurons were increased after 6 days treatment with ethosuximide. Upon b FGF withdrawal, exposure to ethosuximide differentiated the stem cells to MAP 2 positive neural cells (7.18 ± 0.43, 21.766 ± 0.55 and 41.57 ± 0.5 for control, 0.1 and 1 μ m, respectively). GABA immunofluorescence images illustrated that ethosuximide increased GABA ergic neurons (7.19 ± 0.32, 23.23 ± 0.55, and 46.30 ± 0.44 for control, 0.1 and 1 μ m, respectively). Additionally, BrdU immunofluorescence assay showed that ethosuximide‐enhanced nucleus proliferation in the neuronal stem cells. Therefore, the results of this study suggest that ethosuximide may compensate damage caused by seizure attacks and possibly other neuronal loss disorders.