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Elucidation of molecular mechanism involved in neuroprotective effect of C oenzyme Q 10 in alcohol‐induced neuropathic pain
Author(s) -
Kandhare Amit D.,
Ghosh Pinaki,
Ghule Arvindkumar E.,
Bodhankar Subhash L.
Publication year - 2013
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/fcp.12003
Subject(s) - coenzyme q10 , neuropathic pain , pharmacology , chemistry , oxidative stress , antioxidant , vitamin e , medicine , biochemistry , endocrinology
The aim of the present investigation was to evaluate the effect of C oenzyme Q 10 and its combination with vitamin E in alcohol‐induced chronic neuropathic pain. Male W istar rats were orally treated with alcohol (10 g/kg, 35% v/v, b.i.d.) for 10 weeks. Coenzyme Q 10 (25, 50, and 100 mg/kg) and vitamin E (100 mg/kg) were coadministered orally for 1 h after ethanol administration for 10 weeks. Various nerve functions, biochemical, and molecular parameters were assessed. Chronic administration of ethanol for 10 weeks resulted significant development of neuropathic pain. Treatment with C oenzyme Q 10 (50 and 100 mg/kg) for 10 weeks showed significant and dose dependently increased in level of nociceptive threshold, endogenous antioxidant, and N a, K ‐ ATP ase enzyme. Coenzyme Q 10 (50 and 100 mg/kg) significantly restored the levels of motor nerve conduction velocity and sensory nerve conduction velocity. It also showed significant decrease in levels of endogenous calcium, oxidative–nitrosative stress, TNF ‐α, IL ‐1β, and IL ‐4 level. Alteration in protein expression of polymerase gamma (pol γ) was significantly restored the C oenzyme Q 10 treatment. The important finding of the study is that, C oenzyme Q 10 (100 mg/kg) and α‐tocopherol (100 mg/kg) combination‐treated rats showed more significant prevention of behavioral, biochemical, and molecular neurotoxic effect of alcohol administration than C oenzyme Q 10 or α‐tocopherol alone treated group. It is evident from the finding of present investigation that plethora of mechanism including inhibition of oxido‐nitrosative stress, release of pro‐inflammatory cytokine, modulation of endogenous biomarker, and protection of pol γ protein expression simultaneously orchestrate to exhibits neuroprotective effect of C oenzyme Q 10, vitamin E and their combination.