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The effects of human dermal–derived mesenchymal stem cells on the keratinocyte proliferation and apoptosis in psoriasis
Author(s) -
Chang Wenjuan,
Liang Nannan,
Cao Yue,
Xing Jianxiao,
Li Jiao,
Li Juan,
Zhao Xincheng,
Li Junqin,
Niu Xuping,
Hou Ruixia,
Yin Guohua,
Zhang Kaiming
Publication year - 2021
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.14353
Subject(s) - keratinocyte , psoriasis , mesenchymal stem cell , apoptosis , annexin , cancer research , cell growth , proinflammatory cytokine , stem cell , dermis , immunology , inflammation , chemistry , microbiology and biotechnology , biology , medicine , cell culture , pathology , biochemistry , genetics
Psoriasis is a common chronic inflammatory skin disease, characterized by epidermal hyperproliferation. Mesenchymal stem cells (MSCs) regulate inflammation and vascular proliferation in the psoriasis lesions. Whether dermal‐derived mesenchymal stem cells (DMSCs), the main MSCs in the dermis, regulate keratinocyte proliferation and apoptosis remains unknown. In the present study, we assessed the proliferation and apoptosis of keratinocytes cocultured with DMSCs isolated from either normal or psoriatic involved skin. Cell growth and apoptotic rates were determined using Cell Count Kit‐8 and annexin V‐FITC staining, respectively. In addition, EDU kit was also used to measure the rate of keratinocyte proliferation. Our results showed that psoriatic DMSCs (pDMSCs) were more potent than normal DMSCs (nDMSCs) in stimulating keratinocyte proliferation. In contrast, the apoptotic rate and expression levels of caspase‐3 protein were lower in pDMSC‐treated than nDMSC‐treated keratinocytes (p < 0.001). Moreover, significantly higher contents of IL‐6, IL‐8, TNF‐α and IFN‐γ were found in the culture medium of pDMSCs than in that of nDMSCs. In conclusion, pDMSCs were more potent than nDMSCs in stimulation of keratinocyte proliferation and secretion of proinflammatory cytokines, but weaker in promoting apoptosis.