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IL‐23 modulates histamine‐evoked itch and responses of pruriceptors in mice
Author(s) -
Pavlenko Darya,
Funahashi Hideki,
Sakai Kent,
Hashimoto Takashi,
Lozada Taisa,
Yosipovitch Gil,
Akiyama Tasuku
Publication year - 2020
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.14206
Subject(s) - scratching , histaminergic , histamine , trpv1 , dorsal root ganglion , capsaicin , chemistry , calcium , pharmacology , calcium imaging , in vivo , medicine , endocrinology , sensory system , neuroscience , transient receptor potential channel , receptor , psychology , biology , physics , microbiology and biotechnology , acoustics
Accumulating evidence has highlighted the essential roles of cytokines in itch processing. Although IL‐23 and Th17 cytokines are elevated in inflammatory skin disorders, their role in itch is unknown. Here, we investigated the role of IL‐23 and IL‐17A in itch response using an in vitro calcium imaging of mouse dorsal root ganglion (DRG) neurons and an in vivo behaviour test. Calcium imaging studies revealed that a few DRG neurons (~5%) responded to either IL‐23 or IL‐17A. Pretreatment cells with IL‐23 significantly reduced calcium responses to histamine and capsaicin but not chloroquine. Behaviour experiments showed neither IL‐23 nor IL‐17A evoked scratching. IL‐23 significantly decreased histamine‐evoked scratching without affecting chloroquine‐evoked scratching. There was no difference in scratching between IL‐17A‐ and vehicle‐treated groups. These results indicate that IL‐23 might play a role in regulating histaminergic itch via modulation of TRPV1 activity.