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Severe epidermolysis bullosa simplex phenotype caused by codominant mutations p.Ile377Thr in keratin 14 and p.Gly138Glu in keratin 5
Author(s) -
Bchetnia Mbarka,
Allard JeanPascal,
BoucherLafleur AnneMarie,
Cruz Marino Tania,
Dupéré Audrey,
Powell Julie,
McCuaig Catherine,
Bernier MarieÈve,
Laprise Catherine
Publication year - 2020
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.14189
Subject(s) - epidermolysis bullosa simplex , keratin 5 , keratin 14 , epidermolysis bullosa , missense mutation , keratin , keratin 6a , genodermatosis , phenotype , epidermolytic hyperkeratosis , biology , genetics , mutation , population , hyperkeratosis , keratin 8 , dermatology , pathology , gene , medicine , intermediate filament , cell , cytoskeleton , transgene , environmental health , genetically modified mouse
Epidermolysis bullosa simplex (EBS) is a rare skin disease usually inherited in an autosomal dominant pattern. EBS is resulting from mutations in keratin 5 ( KRT5) and keratin 14 ( KRT14) genes encoding the keratins 5 and 14 proteins expressed in the keratinocytes of the basal layer of the epidermis. To date, seven pathogenic mutations have been reported to be responsible for EBS in the Canadian population from the province of Quebec: p.Pro25Leu, p.Leu150Pro, p.Met327Thr and p.Arg559X in KRT5 ; p.Arg125Ser, p.Ile377Thr and p.Ile412Phe in KRT14 . Here, we present a novel French‐Canadian patient diagnosed with EBS confined to the soles but presenting a severe complication form including blisters, hyperkeratosis, skin erosions and toenail abnormalities. Mutation screening was performed by direct sequencing of the entire coding regions of KRT5 and KRT14 genes and revealed the previously reported missense heterozygous mutation c. 1130T > C in KRT14 (p.Ile377Thr). Furthermore, this patient is carrying a second mutation in KRT5 , c.413G > A (p.Gly138Glu), which has been linked to an increased risk of basal cell carcinoma in the literature. We suspect an impact of the p.Gly138Glu variant on the EBS phenotype severity of the studied patient. The pathogenicity and consequences of both genetic variations were simulated by in silico tools.