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TINCR is not a non‐coding RNA but encodes a protein component of cornified epidermal keratinocytes
Author(s) -
Eckhart Leopold,
Lachner Julia,
Tschachler Erwin,
Rice Robert H.
Publication year - 2020
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.14083
Subject(s) - open reading frame , biology , keratinocyte , rna , proteome , long non coding rna , computational biology , messenger rna , coding region , microbiology and biotechnology , genetics , gene , in vitro , peptide sequence
Long non‐coding RNAs have been implicated in the regulation of a plethora of biological processes, yet it has been challenging to verify that they are truly not coding for proteins. Terminal differentiation‐induced non‐coding RNA (TINCR) is a 3.7‐kilobase mRNA that is highly abundant in epidermal keratinocytes prior to cornification. Here, we report the presence of an evolutionarily conserved open reading frame in TINCR and the identification of peptides derived from this open reading frame in the proteome of human stratum corneum. Our results demonstrate that TINCR is a protein‐coding RNA and suggest that the TINCR‐encoded protein is involved in keratinocyte cornification.