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Downregulation of TUG 1 promotes melanogenesis and UVB ‐induced melanogenesis
Author(s) -
Fu Chuhan,
Chen Jing,
Lu Jianyun,
Pei Shiyao,
Hu Shuanghai,
Jiang Ling,
Ding Yufang,
Huang Lihua,
Xiang Hong,
Huang Jinhua,
Zeng Qinghai
Publication year - 2019
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13929
Subject(s) - downregulation and upregulation , phosphorylation , mapk/erk pathway , microbiology and biotechnology , chemistry , gene , biology , biochemistry
Studies have revealed that taurine upregulated gene 1 ( TUG 1), an important member of the long non‐coding RNA family, is involved in the regulation of cell growth, tumorigenesis and invasion, insulin secretion and so on. However, its role in melanogenesis has not been explored. This study attempts to explore the effects of TUG 1 on melanogenesis and its regulatory mechanisms. We evaluated the expression changes in melanogenesis‐related genes and detected phosphorylation levels of ERK , JNK and P38 in TUG 1 downregulated melanocytes. After exposure of melanocytes to UVB irradiation, the expression of TUG 1 and melanogenesis‐related genes was detected. We found that the expression of tyrosinase ( TYR ), tyrosine‐related protein 1 ( TYRP 1) and tyrosine‐related protein 2 ( TYRP 2) was upregulated and that the phosphorylation level of ERK was downregulated by downregulating TUG 1. Inhibition of TUG 1 could further upregulate the expression of UVB ‐induced melanogenesis‐related genes. In conclusion, TUG 1 negatively regulates melanocyte melanogenesis via the ERK pathway and plays a negative role in UVB ‐induced melanogenesis.