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Adipose‐derived stem cells attenuate atopic dermatitis‐like skin lesions in NC /Nga mice
Author(s) -
Park HyunSun,
Son HyeYoun,
Choi MinHa,
Son Youngsook,
Kim Sundong,
Hong HyunSook,
Park JiUng
Publication year - 2019
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13895
Subject(s) - medicine , thymic stromal lymphopoietin , chemokine , adipose tissue , atopic dermatitis , stem cell , immunology , mesenchymal stem cell , immune system , inflammation , pathology , biology , genetics
There is an unmet need in novel therapeutics for atopic dermatitis ( AD ). We examined the effects of autologous adipose‐derived stem cells ( ADSC s) on AD ‐like skin lesions induced by the application of 2,4‐dinitrochlorobenzene ( DNCB ) in NC /Nga mice. Autologous ADSC s and ADSC ‐conditioned medium ( ADSC ‐ CM ) were injected intralesionally three times. Clinical severity and histopathologic findings were compared in sham naïve control, saline‐treated, ADSC ‐treated, ADSC ‐ CM ‐treated and 2.5% cortisone lotion‐applied animals. The severity index, skin thickness, mast cell number, as well as expression levels of thymic stromal lymphopoietin, CD 45, chemoattractant receptor‐homologous molecule, chemokine ligand 9 and chemokine ligand 20 were significantly lower in mice treated with ADSC , ADSC ‐ CM , or 2.5% cortisone lotion. Tissue levels of interferon‐γ as well as serum levels of interleukin‐33 and immunoglobulin E levels were also decreased in those groups. We conclude that autologous ADSC s improved DNCB ‐induced AD ‐like skin lesions in NC /Nga mice by reducing inflammation associated with Th2 immune response and interferon‐γ.