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Patients with congenital ichthyosis and TGM 1 mutations overexpress other ARCI genes in the skin: Part of a barrier repair response?
Author(s) -
Zhang Hanqian,
Ericsson Maja,
Weström Simone,
Vahlquist Anders,
Virtanen Marie,
Törmä Hans
Publication year - 2019
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13813
Subject(s) - congenital ichthyosis , keratinocyte , downregulation and upregulation , biology , gene , microbiology and biotechnology , microarray , microarray analysis techniques , gene expression , proinflammatory cytokine , immunology , genetics , inflammation , cell culture
Autosomal recessive congenital ichthyosis ( ARCI ) is a group of monogenic skin disorders caused by mutations in any of at least 12 different genes, many of which are involved in the epidermal synthesis of ω‐O‐acylceramides (acylCer). AcylCer are essential precursors of the corneocyte lipid envelope crosslinked by transglutaminase‐1 ( TG m‐1), or a yet unidentified enzyme, for normal skin barrier formation. We hypothesized that inactivating TGM 1 mutations will lead to a compensatory overexpression of the transcripts involved in skin barrier repair, including many other ARCI ‐causing genes. Using microarray, we examined the global mRNA expression profile in skin biopsies from five ARCI patients with TGM 1 mutations and four healthy controls. There were a total of 599 significantly differentially expressed genes (adjusted P < 0.05), out of which 272 showed more than 1.5 log2fold‐change ( FC ) up‐ or down‐regulation. Functional classification of the latter group of transcripts showed enrichment of mRNA encoding proteins mainly associated with biological pathways involved in keratinocyte differentiation and immune response. Moreover, the expression of seven out of twelve ARCI ‐causing genes was significantly increased ( FC = 0.98‐2.05). Also, many of the genes involved in keratinocyte differentiation (cornified envelope formation) and immune response (antimicrobial peptides and proinflammatory cytokines) were upregulated. The results from the microarray analysis were also verified for selected genes at the mRNA level by qPCR and at the protein level by semi‐quantitative immunofluorescence. The upregulation of these genes might reflect a compensatory induction of acylCer biosynthesis as a part of a global barrier repair response in the patient′s epidermis.