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On the relevance of an in vitro reconstructed human epidermis model for drug screening in atopic dermatitis
Author(s) -
Hubaux Roland,
Bastin Coralie,
Salmon Michel
Publication year - 2018
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13810
Subject(s) - atopic dermatitis , epidermis (zoology) , dermatology , medicine , in vitro , drug , human skin , relevance (law) , pharmacology , biology , anatomy , biochemistry , genetics , political science , law
Recent advances in the development of human‐based in vitro models offer new tools for drug screening and mechanistic investigations of new therapeutic agents. However, there is a lack of evidence that disease models respond favourably to potential drug candidates. Atopic dermatitis ( AD ) is a very common disease associated with an altered skin barrier and chronic inflammation. Here, we demonstrate that the AD ‐like features of a reconstructed human epidermis ( RHE ) model treated with Th2 cytokines are reversed in the presence of molecules known to have a beneficial effect on damaged skin as a result of modulating various signalling cascades including the Liver X Receptors and JAK / STAT pathways. This work shows that standardized and reproducible RHE are relevant models for therapeutic research assessing new drug candidates aiming to restore epidermal integrity in an inflammatory environment.