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Decorin promotes proliferation and migration of ORS keratinocytes and maintains hair anagen in mice
Author(s) -
Zhou Lijuan,
Jing Jing,
Wang Han,
Wu Xianjie,
Lu Zhongfa
Publication year - 2018
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13770
Subject(s) - decorin , microbiology and biotechnology , wnt signaling pathway , hair follicle , proteoglycan , hair cycle , downregulation and upregulation , keratinocyte , catenin , chemistry , in vitro , biology , extracellular matrix , signal transduction , biochemistry , gene
DECORIN is a prototypical member of the small leucine‐rich proteoglycan ( SLRP ) family that plays important roles in numerous biological processes and cellular biological pathways. We previously showed that Decorin expression was highly enhanced in mouse dorsal hair follicles ( HF s) during the anagen phase and was reduced during the catagen and telogen phases, suggesting that Decorin might modulate follicular cycling and morphogenesis. In this study, to further clarify the effects of DECORIN on hair cells and the cycling transition, an in vitro overexpression strategy and Decorin ‐null ( Dcn −/− ) mice were used to investigate the effects of DECORIN on outer root sheath ( ORS ) keratinocytes. DECORIN overexpression significantly enhanced proliferation and migration in ORS keratinocytes in vitro. Moreover, DECORIN overexpression upregulated the mRNA and protein expression levels of WNT 10b , β ‐ CATENIN and LEF 1 . The DECORIN overexpression‐induced increase in the proliferation and migration of ORS keratinocytes was partially inhibited by a Wnt/β‐catenin inhibitor. Furthermore, Dcn − /− mice had a shortened anagen phase and lower levels of β‐catenin expression than were observed in wild‐type mice in imaging and histological analyses. Taken together, these findings suggest that DECORIN promotes the proliferation and migration of ORS keratinocytes in vitro and maintains hair anagen in mice.