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UVB‐inhibited H19 activates melanogenesis by paracrine effects
Author(s) -
Pei Shiyao,
Huang Jinhua,
Chen Jing,
Hu Shuanghai,
Lei Li,
Fu Chuhan,
Jiang Ling,
Ding Yufang,
Leng Yiming,
Huang Lihua,
Xiang Hong,
Zeng Qinghai
Publication year - 2018
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13749
Subject(s) - paracrine signalling , western blot , downregulation and upregulation , transfection , microbiology and biotechnology , rna , biology , cell culture , melanocyte , chemistry , cancer research , gene , melanoma , biochemistry , receptor , genetics
The long noncoding RNA H19 was reported to associate with melanogenesis. However, it remains unknown whether H19 expression will be changed by UVB irradiation and whether H19 will regulate melanocytes melanogenesis by paracrine effects. Here, we analysed the expression changes of H19 irradiated by UVB in keratinocytes and explored the mechanism of melanogenesis stimulated by H19 through paracrine effects. First, after keratinocytes were exposed to UVB irradiation, expression of H19 and pro‐opiomelanocortin ( POMC ) was measured by qRT ‐ PCR . Also, α‐melanocyte‐stimulating hormone (α‐ MSH ) contents in cells supernatant were measured by ELISA . Then, H19 si RNA s were designed and transfected into keratinocytes by liposome. The expression changes of H19, POMC and α‐ MSH were detected. Besides, expression of p53 was detected by Western blot. After that, supernatant of keratinocytes with H19 si RNA s or negative control si RNA was cocultured with immortalized melanocyte line PIG 1. Expression levels of Mi TF , TYR , Rab27A, TYRP 2, FSCN 1 and MYO 5A in PIG 1 cells were detected by Western blot and qRT ‐ PCR . We found that H19 expression of keratinocytes cells decreased after UVB irradiation. However, the levels of POMC , α‐ MSH and p53 were upregulated in UVB ‐irradiated cells. Compared with the negative control, H19 si RNA s could significantly increase the expression of POMC , α‐ MSH and p53. After supernatant of keratinocytes transfected with H19 si RNA s was cocultured with PIG 1 cells, the levels of Mi TF , TYR and Rab27A were upregulated in PIG 1 cells. In conclusion, UVB ‐inhibited H19 may promote α‐ MSH secretion by p53 in keratinocytes and then regulate melanocytes melanogenesis through paracrine effects.