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Comprehensive circ RNA expression profile and construction of circ RNA ‐associated ce RNA network in fur skin
Author(s) -
Zhu Zhiwei,
Li Yuan,
Liu Wenyan,
He Junping,
Zhang Lihuan,
Li Huifeng,
Li Pengfei,
Lv Lihua
Publication year - 2018
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13502
Subject(s) - rna , messenger rna , rna editing , non coding rna , microbiology and biotechnology , gene expression , biology , transcription (linguistics) , gene , genetics , linguistics , philosophy
Circular RNA (circ RNA ), a class of non‐coding RNA s, is a new group of RNA s that are related to tumorigenesis. The role of circ RNA s in various diseases has been already highlighted. However, the expression levels and functions of circ RNA s related to the melanocytes in the skin are poorly understood. RNA sequence was performed to analyse the expression profiles of circ RNA s in black fur skin and white fur skin during different differentiation stages and investigate the relevant metabolism mechanisms. Differentially expressed circ RNA s were detected using empirical Bayes sequencing ( EBS eq) and then verified through the quantitative real‐time PCR method. The EQS eq analysis of circ RNA s identified 11 downregulated and 32 upregulated circ RNA s in the embryo of black fur skin and white fur skin, as well as 21 downregulated and 17 upregulated circ RNA in the postnatal stage. A circ RNA –micro RNA (mi RNA )–messenger RNA ( mRNA ) network was established to predict the circ RNA targets. Gene ontology ( GO ) analysis and Kyoto Encyclopedia of Genes and Genomes ( KEGG ) pathway analysis were applied to enrich the mRNA data further. Results showed that the specific mRNA s mainly involved in the transcription‐related GO s, especially GO :0042802, GO :0005080 and GO :0032403, demonstrate their specific actions in transcriptional regulation. In the circ RNA –mi RNA – mRNA network, the most enriched GO terms of the mRNA s were pigmentation, protein autophosphorylation and protein complex. Therefore, the circ RNA –mi RNA – mRNA pathway may reveal novel mechanisms for pigmentation, and circ RNA s may serve as candidates in pigmentation.