Premium
Small‐animal PET imaging analysis with [ 18 F]FHBG in a mouse model of HSV1‐tk gene expression in melanoma
Author(s) -
Lee Onseok,
Kim Jaeyoung,
Oh Chilhwan
Publication year - 2018
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13475
Subject(s) - thymidine kinase , melanoma , herpes simplex virus , gene expression , recombinant dna , pet imaging , animal model , gene , medicine , cell culture , virology , microbiology and biotechnology , virus , cancer research , biology , nuclear medicine , positron emission tomography , biochemistry , genetics
The purpose of this study was to establish a small‐animal model for molecular imaging and to acquire basic data on assessing the efficacy of candidate melanoma drugs using small‐animal PET imaging analysis with [ 18 F]FHBG for herpes simplex virus 1‐thymidine kinase (HSV1‐tk) gene expression in a melanoma mouse model. The B16 melanoma cell line was transduced with a recombinant lentiviral vector containing the HSV1‐tk gene and inoculated into the back skin of C57BL/6J mice. [ 18 F]FHBG PET imaging showed better contrast for HSV1‐tk(+) melanomas compared to brain, heart, gall bladder, intestine and kidney than did [ 18 F]FDG PET imaging.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom