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Visualization of dendritic cells’ responses in atopic dermatitis: Preventing effect of emollient
Author(s) -
Mias Céline,
Le Digabel Jimmy,
Filiol Jérôme,
Gontier Etienne,
Gravier Eléonore,
Villaret Aurélie,
Nocera Thérèse,
Questel Emmanuel,
Rossi AnaBeatris,
Redoulès Daniel,
Josse Gwendal
Publication year - 2018
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13470
Subject(s) - atopic dermatitis , suction blister , epidermis (zoology) , atopy , infiltration (hvac) , immunology , langerhans cell , medicine , chemistry , pathology , dermatology , allergy , immune system , materials science , composite material , anatomy
Atopic dermatitis (AD) is a chronic and multifactorial inflammatory skin disease involving various dendritic cells such as epidermal Langerhans cells (LC) and inflammatory dendritic epidermal cells (IDECs). Most of the clinical studies was performed on isolated cells, and thus, it would be useful to characterize directly on the human epidermal tissue the first cellular events occurred during the AD. The suction blister method was used to obtain whole epidermis samples and interstitial cutaneous fluids. Employing multiphoton microscopy, we analyzed the early dynamic behavior of inflammatory cells using Dermatophagoides pteronyssinus atopy patch test (Derp‐APT) and evaluated the effects of emollient pre‐application. Derp‐APT application provoked rapid and strong infiltration of IDECs, and proliferation and activation of LC in the AD subjects’ epidermis. Moreover, emollient pre‐application strengthened the defective skin barrier and had positive effects on inflammatory cells’ behavior, characterized by the complete inhibition of IDEC influx and the presence of immature LC.