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Quantitative image analysis of protein expression and colocalisation in skin sections
Author(s) -
Zhang Hanqian,
Ericsson Maja,
Virtanen Marie,
Weström Simone,
Wählby Carolina,
Vahlquist Anders,
Törmä Hans
Publication year - 2018
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13457
Subject(s) - in situ , protein expression , microbiology and biotechnology , quantitative analysis (chemistry) , staining , immunofluorescence , proximity ligation assay , chemistry , biology , pathology , biochemistry , chromatography , medicine , antibody , immunology , receptor , organic chemistry , gene
Immunofluorescence ( IF ) and in situ proximity ligation assay ( is PLA ) are techniques that are used for in situ protein expression and colocalisation analysis, respectively. However, an efficient quantitative method to analyse both IF and is PLA staining on skin sections is lacking. Therefore, we developed a new method for semi‐automatic quantitative layer‐by‐layer measurement of protein expression and colocalisation in skin sections using the free open‐source software CellProfiler. As a proof of principle, IF and is PLA of ichthyosis‐related proteins TG m‐1 and SDR 9C7 were examined. The results indicate that this new method can be used for protein expression and colocalisation analysis in skin sections.