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Exosomes from human adipose‐derived stem cells promote proliferation and migration of skin fibroblasts
Author(s) -
Choi Eun Wook,
Seo Min Koo,
Woo Eun Young,
Kim Suk Hyung,
Park Eun Joo,
Kim Sue
Publication year - 2018
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13451
Subject(s) - microvesicles , microbiology and biotechnology , adipose tissue , cell growth , exosome , microrna , stem cell , regeneration (biology) , human skin , biology , chemistry , biochemistry , gene , genetics
This study was undertaken to evaluate whether exosomes from human adipose‐derived stem cells (ASC‐exo) can stimulate the regeneration of human dermal fibroblasts (HDFs). Immunoblotting and FACS analyses showed that ASC‐exo was positive for exosome markers. Fluorescence tracking revealed that the contents of ASC‐exo were transferred into the HDFs. ASC‐exo treatment also stimulated the proliferation and migration of HDFs in a dose‐dependent manner. Similarly, the expression levels of genes involved in skin cell proliferation were increased by ASC‐exo. Microarray analysis showed an enrichment of microRNAs that have regenerative function. We suggest that the ASC‐exo can stimulate skin cell proliferation.