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Contribution of CARD 9‐mediated signalling to wound healing in skin
Author(s) -
Kanno Emi,
Kawakami Kazuyoshi,
Tanno Hiromasa,
Suzuki Aiko,
Sato Noriko,
Masaki Airi,
Imamura Ayano,
Takagi Naoyuki,
Miura Takayuki,
Yamamoto Hideki,
Ishii Keiko,
Hara Hiromitsu,
Imai Yoshimichi,
Maruyama Ryoko,
Tachi Masahiro
Publication year - 2017
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13389
Subject(s) - wound healing , chemokine , cytokine , microbiology and biotechnology , chemistry , receptor , immunology , macrophage , inflammation , biology , biochemistry , in vitro
Abstract The inflammatory response after skin injury involves the secretion of a variety of cytokines and growth factors that are necessary for tissue repair. Caspase recruitment domain‐containing protein 9 ( CARD 9) is an essential signalling adaptor molecule for NF ‐κB activation upon triggering through C‐type lectin receptors ( CLR s), which are expressed in macrophages and dendritic cells. However, the role of CARD 9 in inflammatory responses at the wound site has not been elucidated. In this study, we analysed the role of CARD 9 in the healing process of skin wounds. Wounds were created on the backs of wild‐type ( WT ) C57 BL /6 mice and CARD 9 gene‐disrupted (knockout [ KO ]) mice. We analysed per cent wound closure, and the wound tissues were harvested for analysis of leucocyte accumulation and cytokine and chemokine expressions. CARD 9 KO mice exhibited significant attenuation of wound closure compared with WT mice on days 5, 7 and 10 postwounding, which was associated with decreased macrophage accumulation and reduced TNF ‐α, IL ‐1β, CCL 3 and CCL 4 expressions. These results suggest that CARD 9 may be involved in the wound‐healing process through the regulation of macrophage‐mediated inflammatory responses.