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An efficient method for gene knock‐down by RNA interference in human skin mast cells
Author(s) -
Hazzan Tarek,
Guhl Sven,
Artuc Metin,
Franke Kristin,
Worm Margitta,
Zuberbier Torsten,
Babina Magda
Publication year - 2017
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13358
Subject(s) - transfection , rna interference , rna , gene expression , microbiology and biotechnology , gene , mast cell , biology , genetics , immunology
Mast cells ( MC s) from human skin have been notoriously resistant to gene manipulation, and a method to knock‐down gene expression in in situ differentiated MC s is highly desired. The Dharmacon Accell ® transfection system proved successful on several “difficult‐to‐transfect” cells. In the present work, we therefore tested this method on skin‐derived MC s using different si RNA entities. The si RNA was readily taken up, followed by pronounced, specific reduction of gene and protein expression. Hence, we present the first efficient technique for the manipulation of gene expression in primary skin MC s ex vivo, which combines high transfection rates with retained cell viability.