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CD 4 + TCR γδ + FoxP3 + cells: An unidentified population of immunosuppressive cells towards disease progression leprosy patients
Author(s) -
Tarique Mohd,
Naqvi Raza A.,
Ali Riyasat,
Khaneena,
Rao Donthamshetty Nageswara
Publication year - 2017
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13302
Subject(s) - foxp3 , t cell receptor , il 2 receptor , antigen , population , mycobacterium leprae , biology , immunology , t cell , microbiology and biotechnology , cancer research , immune system , medicine , leprosy , environmental health
This study, for the first time, reveals the role of M. leprae ‐specific CD 4 + TCR γδ + FoxP3 + cells in the progression and pathogenesis of leprosy. Co‐culture with CD 4 + CD 25 − cells suggested the immunosuppressive nature of CD 4 + TCR γδ + cells in dose‐dependent manner. Isolation of CD 4 + TCR γδ + cells from leprosy patients and then culture in presence of M. leprae cell wall antigens ( MLC wA) along with TGF β, IPP and IL ‐2 suggested that these cells are M. leprae specific. TGF ‐β‐mediated SMAD 3 signalling was turned out to be major factor towards the expression of FoxP3 in these cells. SMAD 3 silencing during induction of these cells barely showed the induction of FoxP3. High density of SMAD 3 binding at TGF β RII in CD 4 + TCR γδ + FoxP3 + furthermore suggested the TGF ‐β‐directed SMAD 3 signalling in these cells. Taken together the above data, we can conclude that CD 4 + TCR γδ + FoxP3 + cells possess the potential to track the severity of the disease in leprosy patients.