Premium
Ultraviolet B irradiation increases keratin 1 and keratin 10 expressions in HaCaT keratinocytes via TRPV 1 activation and ERK phosphorylation
Author(s) -
Huang KuoFeng,
Ma KuoHsing,
Liu PeiShan,
Chen BoWei,
Chueh SheauHuei
Publication year - 2017
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13292
Subject(s) - hacat , keratinocyte , phosphorylation , mapk/erk pathway , epidermal growth factor , keratin , keratinocyte growth factor , microbiology and biotechnology , chemistry , kinase , biology , growth factor , biochemistry , receptor , in vitro , paleontology
In this study, we characterized the effect of ultraviolet B ( UVB ) irradiation with or without epidermal growth factor ( EGF ) on the regulation of keratinocyte differentiation under physiological concentration of Ca 2+ (1.8 mM ). In addition, growth factor deprivation used to measure signal transduction and kinase phosphorylation in many studies is physiologically unreal. Therefore, 1% of serum was also included in all experiment. We found that UVB irradiation Ca 2+ dependently induced morphological differentiation and increased keratin 1 and 10 (K1/K10) expressions. Both were inhibited by treatment of cells with EGF . In quiescent cells, phosphorylation of ERK was stimulated by acute EGF treatment, while it rapidly desensitized in chronic EGF treatment or 1% serum exposure. UVB irradiation‐induced keratinocyte differentiation required Ca 2+ influx through TRPV 1. Ca 2+ ‐dependent phosphorylation of ERK was responsible for the expression of K1/10. Cotreatment of cells with EGF during UVB irradiation inhibits the UVB irradiation‐induced differentiation by desensitizing ERK phosphorylation.