Premium
Wy14643, an agonist for PPAR α, downregulates expression of TARC and RANTES in cultured human keratinocytes
Author(s) -
Zhang Wei,
Sakai Takashi,
Fujiwara Sakuhei,
Hatano Yutaka
Publication year - 2017
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13245
Subject(s) - chemokine , microbiology and biotechnology , agonist , receptor , chemokine receptor , downregulation and upregulation , beta defensin , antimicrobial peptides , immunology , inflammation , biology , chemistry , peptide , gene , biochemistry
Beneficial effects of Wy14643, an agonist for peroxisome proliferator‐activated receptor ( PPAR )α, on permeability barrier homeostasis‐related functions of keratinocytes such as up‐regulation of epidermal differentiation‐related molecules and lipid synthesis, have been demonstrated. The present study demonstrated that Wy14643 reduced the expression of thymus and activation‐related chemokine ( TARC ) and regulated on activation normal T cell expressed ( RANTES ) in both single‐ and 3D‐cultured human keratinocytes. The combined data of the present and previous studies support the notion that Wy14643 could be a therapeutic agent that might simultaneously and directly modulate permeability barrier dysfunction and allergic inflammation in the pathogenesis of atopic dermatitis. As for the anti‐microbial barrier function, the present study demonstrated that Wy14643 up‐regulated expression of the anti‐microbial peptide, human β defensin 3, in cultured human keratinocytes only in mRNA levels but not in protein ones, suggesting that Wy14643 might not directly account for the up‐regulation of the anti‐microbial peptide which has been reported in vivo.