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Inhibitory effects of dietary soyasaponin on 2,4‐dinitrofluorobenzene‐induced contact hypersensitivity in mice
Author(s) -
Nagano Takao,
Katase Mitsuru,
Tsumura Kazunobu,
Saito Mineki,
Matsuda Tsukasa
Publication year - 2017
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.13205
Subject(s) - chemokine , receptor , chemistry , pharmacology , inflammation , ratón , immunology , medicine , biochemistry
Soyasaponins ( SS s) abundant in soybean have anti‐inflammatory activities; however, their therapeutic effects on allergic contact dermatitis ( ACD ) remain unknown. To assess the effects of SS ‐enriched diets on ACD , we used a mouse model of contact hypersensitivity ( CHS ). Mice were fed low‐dose or high‐dose SS ‐containing diets for 3 weeks prior to CHS induction with 2,4‐dinitrofluorobenzene ( DNFB ). The low‐dose SS diet attenuated DNFB ‐induced ear swelling and tissue oedema, and reduced the number of infiltrating Gr‐1‐positive myeloid cells. Low‐dose, but not high‐dose, SS s decreased chemokine (C‐X‐C motif) ligand 2 ( CXCL 2) and triggering receptor expressed on myeloid cells ( TREM )‐1 production in ear tissues, compared to a control. Taxonomic 16S rRNA analysis revealed significant alterations in faecal microbiota caused by CHS , which were reversed by low‐dose SS s. The low‐dose SS and non‐ CHS groups clustered together, while the high‐dose SS group split between CHS and non‐ CHS clusters. Our results demonstrated that low‐dose SS s alleviated CHS symptoms by attenuating inflammation and improving the intestinal microbiota composition, suggesting that dietary SS s may have beneficial effects on ACD .