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Photosensitivity of murine skin greatly depends on the genetic background: clinically relevant dose as a new measure to replace minimal erythema dose in mouse studies
Author(s) -
Gyöngyösi Nóra,
Lőrincz Kende,
Keszeg András,
Haluszka Dóra,
Bánvölgyi András,
Tátrai Erika,
Kárpáti Sarolta,
Wikonkál Norbert M.
Publication year - 2016
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12984
Subject(s) - erythema , photosensitivity , hairless , in vivo , dermatology , ex vivo , melanin , medicine , pathology , biology , biochemistry , genetics , materials science , optoelectronics
Artificial UV irradiation of murine skin is a frequently used method for testing photosensitivity, study carcinogenesis and photoprotective effects of different compounds. However, doses of UV radiation and mouse strains used in experiments vary greatly. The genetic background of mice may influence the photosensitivity as melanin content, pigmentation and hair cycle parameters are dissimilar. Doses of UV are often expressed in relation to the minimal erythema dose ( MED ) that was not necessarily determined for the given strain. We set out to standardize the method of measuring photosensitivity in three commonly used mouse strains, C57 BL /6N, Balb/c and SKH ‐1. We found that MED may not be determined for some strains as erythema development in mice with diverse genotypes differs greatly. We measured the oedema response in vivo and ex vivo by using OCT . Given the strain‐specific variability of erythema, we introduced Clinically Relevant Dose ( CRD ) as a new term to replace MED in experiments, to describe the lowest dose that triggers a perceptible skin reaction in mice. Not only the CRD but the proportion of erythema and oedema were different in strains examined. C57 BL /6N mice display skin reactions at the lowest UVB dose, while SKH ‐1 hairless mice show changes, mostly oedema, after higher doses of UVB . The cellular composition and skin thickness were examined by histopathology. IL ‐1beta and IL ‐6 levels in skin correlated with the increasing doses of UVB . Despite the variations in the degree of erythema and oedema, no major differences in cytokine expressions were seen among various strains of mice.