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A three‐dimensional skin equivalent reflecting some aspects of in vivo aged skin
Author(s) -
Diekmann Johanna,
Alili Lirija,
Scholz Okka,
Giesen Melanie,
Holtkötter Olaf,
Brenneisen Peter
Publication year - 2016
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12866
Subject(s) - in vivo , skin equivalent , skin aging , human skin , ageing , in vitro , fibroblast , epidermis (zoology) , elastin , dermal fibroblast , chemistry , pathology , keratinocyte , medicine , biology , dermatology , anatomy , biochemistry , genetics , microbiology and biotechnology
Human skin undergoes morphological, biochemical and functional modifications during the ageing process. This study was designed to produce a 3‐dimensional (3D) skin equivalent in vitro reflecting some aspects of in vivo aged skin. Reconstructed skin was generated by co‐culturing skin fibroblasts and keratinocytes on a collagen–glycosaminoglycan–chitosan scaffold, and ageing was induced by the exposition of fibroblasts to Mitomycin‐C ( MMC ). Recently published data showed that MMC treatment resulted in a drug‐induced accelerated senescence ( DIAS ) in human dermal fibroblast cultures. Next to established ageing markers, histological changes were analysed in comparison with in vivo aged skin. In aged epidermis, the filaggrin expression is reduced in vivo and in vitro . Furthermore, in dermal tissue, the amount of elastin and collagen is lowered in aged skin in vivo as well as after the treatment of 3D skin equivalents with MMC in vitro . Our results show histological signs and some aspects of ageing in a 3D skin equivalent in vitro , which mimics aged skin in vivo .