Premium
miR‐137 inhibits proliferation of melanoma cells by targeting PAK 2
Author(s) -
Hao Shuai,
Luo Chonglin,
Abukiwan Alia,
Wang Guangxia,
He Jinjun,
Huang Lingyun,
Weber Claudia E. M.,
Lv Na,
Xiao Xueyuan,
Eichmüller Stefan B.,
He Dacheng
Publication year - 2015
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12812
Subject(s) - gene knockdown , microrna , melanoma , cell growth , cancer research , western blot , proteomics , biology , in vitro , small interfering rna , cell culture , microbiology and biotechnology , chemistry , transfection , gene , biochemistry , genetics
Micro RNA s (mi RNA ) are key players in a variety of cancers including malignant melanoma. miR‐137 has been reported to be a tumor suppressor in melanoma and several targets have been identified for this mi RNA . We previously developed a novel proteomics technology, 35 S in vivo/vitro labelling analysis for dynamic proteomics (Si LAD ). Because of its high sensitivity in analysing protein expression rates, Si LAD has the potential to unravel mi RNA effects on mRNA s coding for proteins with long half‐lives or high abundance. Using Si LAD , we discovered that miR‐137 significantly downregulated the expression rate of p21‐activated kinase 2 ( PAK 2) in melanoma cells. Bioinformatics analysis predicted PAK 2 as a direct target of miR‐137, which was confirmed by luciferase reporter assay and Western blot analysis. We found that overexpression of miR‐137 inhibited the proliferation of melanoma cells, which could be phenocopied by knockdown of PAK 2 using si RNA s. Furthermore, overexpression of PAK 2 restored miR‐137‐mediated suppression of cell proliferation. These findings indicate that miR‐137 could inhibit proliferation through targeting PAK 2 in melanoma cells.