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Senescent dermal fibroblasts enhance stem cell migration through CCL 2/ CCR 2 axis
Author(s) -
Ohgo Shiro,
Hasegawa Seiji,
Hasebe Yuichi,
Mizutani Hiroshi,
Nakata Satoru,
Akamatsu Hirohiko
Publication year - 2015
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12701
Subject(s) - stem cell , microbiology and biotechnology , fibroblast , dermis , chemokine , dermal fibroblast , stem cell factor , ccl2 , cell , cell migration , chemistry , biology , progenitor cell , receptor , cell culture , anatomy , biochemistry , genetics
During aging, increases in the number of senescent cells are seen in various tissues. On the other hand, stem cells play crucial roles in tissue repair and homeostasis. Therefore, it is likely that stem cells give rise to new cells that replace senescent cells. However, how stem cells contribute to homeostasis in the dermis has not been elucidated. Here, we investigated the effects of factors secreted from senescent fibroblasts on stem cells. We found that senescent human dermal fibroblast ( HDF ) conditioned medium ( CM ) significantly enhanced stem cell migration compared with young HDF CM . The senescent HDF CM strongly secreted chemokine ligand 2 ( CCL 2). Furthermore, CCL 2 was found to enhance stem cell migration, and the inhibition of CCR 2, a receptor for CCL 2, reduced stem cell migration. These results suggest that senescent fibroblasts recruit stem cells by secreting various factors and that the CCL 2/ CCR 2 axis is one of the mechanisms underlying this phenomenon.