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Restorative effect of hair follicular dermal cells on injured human hair follicles in a mouse model
Author(s) -
Yamao Mikaru,
Inamatsu Mutsumi,
Okada Taro,
Ogawa Yuko,
Ishida Yuji,
Tateno Chise,
Yoshizato Katsutoshi
Publication year - 2015
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12625
Subject(s) - dermal papillae , in vivo , scalp , hair follicle , vellus hair , transplantation , dermal fibroblast , medicine , dermis , pathology , chemistry , dermatology , biology , in vitro , surgery , endocrinology , fibroblast , microbiology and biotechnology , biochemistry
No model is available for examining whether in vivo ‐damaged human hair follicles (hu‐ HF s) are rescued by transplanting cultured hu‐ HF dermal cells (dermal papilla and dermal sheath cells). Such a model might be valuable for examining whether in vivo ‐damaged hu‐ HF s such as miniaturized hu‐ HF s in androgenic alopecia are improvable by auto‐transplanting hu‐ HF dermal cells. In this study, we first developed mice with humanized skin composed of hu‐keratinocytes and hu‐dermal fibroblasts. Then, a ‘humanized scalp model mouse’ was generated by transplanting hu‐scalp HF s into the humanized skin. To demonstrate the usability of the model, the lower halves of the hu‐ HF s in the model were amputated in situ, and cultured hu‐ HF dermal cells were injected around the amputated area. The results demonstrated that the transplanted cells contributed to the restoration of the damaged HF s. This model could be used to explore clinically effective technologies for hair restoration therapy by autologous cell transplantation.