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Laser capture microdissection followed by next‐generation sequencing identifies disease‐related micro RNA s in psoriatic skin that reflect systemic micro RNA changes in psoriasis
Author(s) -
Løvendorf Marianne B.,
Mitsui Hiroshi,
Zibert John R.,
Røpke Mads A.,
Hafner Markus,
DyringAndersen Beatrice,
Bonefeld Charlotte M.,
Krueger James G.,
Skov Lone
Publication year - 2015
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1111/exd.12604
Subject(s) - psoriasis , laser capture microdissection , microrna , peripheral blood mononuclear cell , pathogenesis , rna , biology , immune system , dermis , immunology , medicine , pathology , cancer research , gene expression , gene , genetics , in vitro
Psoriasis is a systemic disease with cutaneous manifestations. Micro RNA s (mi RNA s) are small non‐coding RNA molecules that are differentially expressed in psoriatic skin; however, only few cell‐ and region‐specific mi RNA s have been identified in psoriatic lesions. We used laser capture microdissection ( LCM ) and next‐generation sequencing ( NGS ) to study the specific mi RNA expression profiles in the epidermis (Epi) and dermal inflammatory infiltrates ( RD ) of psoriatic skin ( N = 6). We identified 24 deregulated mi RNA s in the Epi and 37 deregulated mi RNA s in the RD of psoriatic plaque compared with normal psoriatic skin ( FCH > 2, FDR < 0.05). Interestingly, 9 of the 37 mi RNA s in RD , including miR‐193b and miR‐223, were recently described as deregulated in circulating peripheral blood mononuclear cells ( PBMC s) from patients with psoriasis. Using flow cytometry and qRT ‐ PCR , we found that miR‐193b and miR‐223 were expressed in Th17 cells. In conclusion, we demonstrate that LCM combined with NGS provides a robust approach to explore the global mi RNA expression in the epidermal and dermal compartments of psoriatic skin. Furthermore, our results indicate that the altered local mi RNA changes seen in the RD are reflected in the circulating immune cells, suggesting that mi RNA s may contribute to the pathogenesis of psoriasis.